Composite

Part:BBa_K3329064:Design

Designed by: Wenfei Xiao   Group: iGEM20_BNDS_China   (2020-10-20)


SpyCatcher003*3-CBM*2-mRFP for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 692
    Illegal BglII site found at 744
    Illegal XhoI site found at 144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1105
    Illegal AgeI site found at 2047
    Illegal AgeI site found at 2314
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 390


Design Notes

Optimized for E. coli


Source

From Article///

References